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Administration Basic Sciences Clinical Sciences Centers of Excellence

 

Haydee  E. P.  Bazan , Ph.D.

Professor, Ophthalmology, Biochemistry and Molecular Biology and Neuroscience

LSU Neuroscience Center of Excellence
2020 Gravier St. Suite D
New Orleans, LA 70112-2234
Phone (office): (504) 599-0877
Phone (Lab): 
Fax: (504) 568-2385
Email: hbazan1@lsuhsc.edu

Research Interests

Lipids Involved in Signal Transduction Mechanisms in the Eye: Some of Dr. HEP Bazan's laboratory studies lipid second messengers in the retina and cornea, particularly platelet activating factor (PAF) a potent lipid mediator of inflammatory and immunological responses. Dr. Bazan's laboratory has demonstrated that PAF, is generated in retina and cornea after injury (e.g., laser photocoagulation, chemical burns in the eye). PAF mediates its action through receptors and Dr. Bazan's laboratory has discovered that this particular lipid induces the expression of genes involved in the degradation of components of the extracellular matrix (ECM) that are very important in the process of tissue repair. PAF also induces the expression of genes such as cyclooxygenase-2 (COX-2), increasing the synthesis of prostaglandins and producing sustained inflammation in the eye.

Another research interest is the study of cell signal transduction events during corneal wound healing, particularly how specific growth factors that are released during injury activate kinase to communicate their signals from the cell membrane to the nuclei. Several animal models, as well as cells and organ cultures, are used. Analytical procedures such as high performance and fast performance-liquid chromatography (HPLC and FPLC) and gas liquid-chromatography (GLC) and a variety of laboratory techniques such as Western and Northern blot, PCR, immunoprecipitations, kinase assays and Ca2+ imaging are employed. The laboratories are on the 8th floor of the Lions Building.

Selected Publications
1. Esquenazi S. He J, Bazan HEP, Bazan NG. Prevention of experimental diffuse lamellar keratitis (DLK) by a novel platelet-activating factor receptor antagonist. J Cataract Refract. Surg (in press).
2. Sharma GF, He J, Bazan HEP. P38 and ERK1/2 coordinate cellular migration and proliferation in epithelial wound healing. Evidence of cross-talk activation between MAP kinase cascades. J. Biol. Chem. 278(24):21989-21997, 2003.
3. Ottino P, Taheri F, Bazan HEP. Growth factor- induced proliferation in corneal epithelial cells is mediated by 12(S)-HETE. Exp. Eye Res. 76(5):613-622, 2003
4. Parker MA, Bazan HEP, Marcheselli VL, Rodriguez de Turco EB, Bazan NG. Platelet-activating factor induces permeability transition and cytochrome c release in isolated brain mitochondria. J. Neurosci. Res. 68:39-50, 2002.
5. Chandraseker G, Ma X, Lallier TE, Bazan HEP. Delay of corneal epithelial wound healing and induction of keratocyte apoptosis by platelet activating factor (PAF). Invest. Ophthalmol. Vis. Sci. 43:1422-28, 2002.
6. Ottino P, Taheri F, Bazan HEP. Platelet-activating factor induces the gene expression of TIMP-1, TIMP-2, and PAI-1: Imbalance between the gene expression of MMP-9 and TIMP-1 and -2. Exp. Eye Res. 74(3):393-402, 2002.